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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Scientific Method

Illustration by J.R. Bee. ThoughtCo. 

• Cell Biology
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• B.A., Biology, Emory University
• A.S., Nursing, Chattahoochee Technical College

The scientific method is a series of steps followed by scientific investigators to answer specific questions about the natural world. It involves making observations, formulating a hypothesis , and conducting scientific experiments . Scientific inquiry starts with an observation followed by the formulation of a question about what has been observed. The steps of the scientific method are as follows:

Observation

The first step of the scientific method involves making an observation about something that interests you. This is very important if you are doing a science project because you want your project to be focused on something that will hold your attention. Your observation can be on anything from plant movement to animal behavior, as long as it is something you really want to know more about.​ This is where you come up with the idea for your science project.

Once you've made your observation, you must formulate a question about what you have observed. Your question should tell what it is that you are trying to discover or accomplish in your experiment. When stating your question you should be as specific as possible.​ For example, if you are doing a project on plants , you may want to know how plants interact with microbes. Your question may be: Do plant spices inhibit bacterial growth ?

The hypothesis is a key component of the scientific process. A hypothesis is an idea that is suggested as an explanation for a natural event, a particular experience, or a specific condition that can be tested through definable experimentation. It states the purpose of your experiment, the variables used, and the predicted outcome of your experiment. It is important to note that a hypothesis must be testable. That means that you should be able to test your hypothesis through experimentation .​ Your hypothesis must either be supported or falsified by your experiment. An example of a good hypothesis is: If there is a relation between listening to music and heart rate, then listening to music will cause a person's resting heart rate to either increase or decrease.

Once you've developed a hypothesis, you must design and conduct an experiment that will test it. You should develop a procedure that states very clearly how you plan to conduct your experiment. It is important that you include and identify a controlled variable or dependent variable in your procedure. Controls allow us to test a single variable in an experiment because they are unchanged. We can then make observations and comparisons between our controls and our independent variables (things that change in the experiment) to develop an accurate conclusion.​

The results are where you report what happened in the experiment. That includes detailing all observations and data made during your experiment. Most people find it easier to visualize the data by charting or graphing the information.​

The final step of the scientific method is developing a conclusion. This is where all of the results from the experiment are analyzed and a determination is reached about the hypothesis. Did the experiment support or reject your hypothesis? If your hypothesis was supported, great. If not, repeat the experiment or think of ways to improve your procedure.

• Six Steps of the Scientific Method
• What Is an Experiment? Definition and Design
• Scientific Method Flow Chart
• Scientific Method Lesson Plan
• How To Design a Science Fair Experiment
• Science Projects for Every Subject
• How to Do a Science Fair Project
• What Are the Elements of a Good Hypothesis?
• How to Write a Lab Report
• What Is a Hypothesis? (Science)
• Biology Science Fair Project Ideas
• Understanding Simple vs Controlled Experiments
• Null Hypothesis Definition and Examples
• Stove Top Frozen Pizza Science Experiment
• Dependent Variable Definition and Examples
• What Is the Difference Between Hard and Soft Science?

Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

• Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis, and it must be possible to prove your hypothesis true or false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

• No. This statement is not testable or falsifiable.
• No. This statement is not testable.
• No. This statement is not falsifiable.
• Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

• Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
• There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
• Some types of HPV can cause cervical cancer.
• About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

• HPV causes cervical cancer.
• People should not have unprotected sex with many partners.
• People who get the vaccine will not get HPV.
• The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

• This group is given a placebo.
• This group is deliberately infected with HPV.
• This group is given nothing.
• This group is given the HPV vaccine.
• a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
• d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

• Sex (all of the subjects will be female)
• Presence or absence of the HPV vaccine
• Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

• Sex (male or female)
• Rates of HPV infection
• Age (years)
• Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
• Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

The Scientific Method by Science Made Simple

Understanding and using the scientific method.

The Scientific Method is a process used to design and perform experiments. It's important to minimize experimental errors and bias, and increase confidence in the accuracy of your results.

In the previous sections, we talked about how to pick a good topic and specific question to investigate. Now we will discuss how to carry out your investigation.

Steps of the Scientific Method

• Observation/Research
• Experimentation

Now that you have settled on the question you want to ask, it's time to use the Scientific Method to design an experiment to answer that question.

If your experiment isn't designed well, you may not get the correct answer. You may not even get any definitive answer at all!

The Scientific Method is a logical and rational order of steps by which scientists come to conclusions about the world around them. The Scientific Method helps to organize thoughts and procedures so that scientists can be confident in the answers they find.

OBSERVATION is first step, so that you know how you want to go about your research.

HYPOTHESIS is the answer you think you'll find.

PREDICTION is your specific belief about the scientific idea: If my hypothesis is true, then I predict we will discover this.

EXPERIMENT is the tool that you invent to answer the question, and

CONCLUSION is the answer that the experiment gives.

Don't worry, it isn't that complicated. Let's take a closer look at each one of these steps. Then you can understand the tools scientists use for their science experiments, and use them for your own.

OBSERVATION

This step could also be called "research." It is the first stage in understanding the problem.

After you decide on topic, and narrow it down to a specific question, you will need to research everything that you can find about it. You can collect information from your own experiences, books, the internet, or even smaller "unofficial" experiments.

Let's continue the example of a science fair idea about tomatoes in the garden. You like to garden, and notice that some tomatoes are bigger than others and wonder why.

Because of this personal experience and an interest in the problem, you decide to learn more about what makes plants grow.

For this stage of the Scientific Method, it's important to use as many sources as you can find. The more information you have on your science fair topic, the better the design of your experiment is going to be, and the better your science fair project is going to be overall.

Also try to get information from your teachers or librarians, or professionals who know something about your science fair project. They can help to guide you to a solid experimental setup.

The next stage of the Scientific Method is known as the "hypothesis." This word basically means "a possible solution to a problem, based on knowledge and research."

The hypothesis is a simple statement that defines what you think the outcome of your experiment will be.

All of the first stage of the Scientific Method -- the observation, or research stage -- is designed to help you express a problem in a single question ("Does the amount of sunlight in a garden affect tomato size?") and propose an answer to the question based on what you know. The experiment that you will design is done to test the hypothesis.

Using the example of the tomato experiment, here is an example of a hypothesis:

TOPIC: "Does the amount of sunlight a tomato plant receives affect the size of the tomatoes?"

HYPOTHESIS: "I believe that the more sunlight a tomato plant receives, the larger the tomatoes will grow.

This hypothesis is based on:

(1) Tomato plants need sunshine to make food through photosynthesis, and logically, more sun means more food, and;

(2) Through informal, exploratory observations of plants in a garden, those with more sunlight appear to grow bigger.

The hypothesis is your general statement of how you think the scientific phenomenon in question works.

Your prediction lets you get specific -- how will you demonstrate that your hypothesis is true? The experiment that you will design is done to test the prediction.

An important thing to remember during this stage of the scientific method is that once you develop a hypothesis and a prediction, you shouldn't change it, even if the results of your experiment show that you were wrong.

An incorrect prediction does NOT mean that you "failed." It just means that the experiment brought some new facts to light that maybe you hadn't thought about before.

Continuing our tomato plant example, a good prediction would be: Increasing the amount of sunlight tomato plants in my experiment receive will cause an increase in their size compared to identical plants that received the same care but less light.

This is the part of the scientific method that tests your hypothesis. An experiment is a tool that you design to find out if your ideas about your topic are right or wrong.

It is absolutely necessary to design a science fair experiment that will accurately test your hypothesis. The experiment is the most important part of the scientific method. It's the logical process that lets scientists learn about the world.

On the next page, we'll discuss the ways that you can go about designing a science fair experiment idea.

The final step in the scientific method is the conclusion. This is a summary of the experiment's results, and how those results match up to your hypothesis.

You have two options for your conclusions: based on your results, either:

(1) YOU CAN REJECT the hypothesis, or

(2) YOU CAN NOT REJECT the hypothesis.

This is an important point!

You can not PROVE the hypothesis with a single experiment, because there is a chance that you made an error somewhere along the way.

What you can say is that your results SUPPORT the original hypothesis.

If your original hypothesis didn't match up with the final results of your experiment, don't change the hypothesis.

Instead, try to explain what might have been wrong with your original hypothesis. What information were you missing when you made your prediction? What are the possible reasons the hypothesis and experimental results didn't match up?

Remember, a science fair experiment isn't a failure simply because does not agree with your hypothesis. No one will take points off if your prediction wasn't accurate. Many important scientific discoveries were made as a result of experiments gone wrong!

A science fair experiment is only a failure if its design is flawed. A flawed experiment is one that (1) doesn't keep its variables under control, and (2) doesn't sufficiently answer the question that you asked of it.

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Learning Objectives

• Identify the components of the scientific method.

Scientists search for answers to questions and solutions to problems by using a procedure called the scientific method. This procedure consists of making observations, formulating hypotheses, and designing experiments; which leads to additional observations, hypotheses, and experiments in repeated cycles (Figure \(\PageIndex{1}\)).

Step 1: Make observations

Observations can be qualitative or quantitative. Qualitative observations describe properties or occurrences in ways that do not rely on numbers. Examples of qualitative observations include the following: "the outside air temperature is cooler during the winter season," "table salt is a crystalline solid," "sulfur crystals are yellow," and "dissolving a penny in dilute nitric acid forms a blue solution and a brown gas." Quantitative observations are measurements, which by definition consist of both a number and a unit. Examples of quantitative observations include the following: "the melting point of crystalline sulfur is 115.21° Celsius," and "35.9 grams of table salt—the chemical name of which is sodium chloride—dissolve in 100 grams of water at 20° Celsius." For the question of the dinosaurs’ extinction, the initial observation was quantitative: iridium concentrations in sediments dating to 66 million years ago were 20–160 times higher than normal.

Step 2: Formulate a hypothesis

After deciding to learn more about an observation or a set of observations, scientists generally begin an investigation by forming a hypothesis, a tentative explanation for the observation(s). The hypothesis may not be correct, but it puts the scientist’s understanding of the system being studied into a form that can be tested. For example, the observation that we experience alternating periods of light and darkness corresponding to observed movements of the sun, moon, clouds, and shadows is consistent with either one of two hypotheses:

• Earth rotates on its axis every 24 hours, alternately exposing one side to the sun.
• The sun revolves around Earth every 24 hours.

Suitable experiments can be designed to choose between these two alternatives. For the disappearance of the dinosaurs, the hypothesis was that the impact of a large extraterrestrial object caused their extinction. Unfortunately (or perhaps fortunately), this hypothesis does not lend itself to direct testing by any obvious experiment, but scientists can collect additional data that either support or refute it.

Step 3: Design and perform experiments

After a hypothesis has been formed, scientists conduct experiments to test its validity. Experiments are systematic observations or measurements, preferably made under controlled conditions—that is—under conditions in which a single variable changes.

Step 4: Accept or modify the hypothesis

A properly designed and executed experiment enables a scientist to determine whether or not the original hypothesis is valid. If the hypothesis is valid, the scientist can proceed to step 5. In other cases, experiments often demonstrate that the hypothesis is incorrect or that it must be modified and requires further experimentation.

Step 5: Development into a law and/or theory

More experimental data are then collected and analyzed, at which point a scientist may begin to think that the results are sufficiently reproducible (i.e., dependable) to merit being summarized in a law, a verbal or mathematical description of a phenomenon that allows for general predictions. A law simply states what happens; it does not address the question of why.

One example of a law, the law of definite proportions , which was discovered by the French scientist Joseph Proust (1754–1826), states that a chemical substance always contains the same proportions of elements by mass. Thus, sodium chloride (table salt) always contains the same proportion by mass of sodium to chlorine, in this case 39.34% sodium and 60.66% chlorine by mass, and sucrose (table sugar) is always 42.11% carbon, 6.48% hydrogen, and 51.41% oxygen by mass.

Whereas a law states only what happens, a theory attempts to explain why nature behaves as it does. Laws are unlikely to change greatly over time unless a major experimental error is discovered. In contrast, a theory, by definition, is incomplete and imperfect, evolving with time to explain new facts as they are discovered.

Because scientists can enter the cycle shown in Figure \(\PageIndex{1}\) at any point, the actual application of the scientific method to different topics can take many different forms. For example, a scientist may start with a hypothesis formed by reading about work done by others in the field, rather than by making direct observations.

Example \(\PageIndex{1}\)

Classify each statement as a law, a theory, an experiment, a hypothesis, an observation.

• Ice always floats on liquid water.
• Birds evolved from dinosaurs.
• Hot air is less dense than cold air, probably because the components of hot air are moving more rapidly.
• When 10 g of ice were added to 100 mL of water at 25°C, the temperature of the water decreased to 15.5°C after the ice melted.
• The ingredients of Ivory soap were analyzed to see whether it really is 99.44% pure, as advertised.
• This is a general statement of a relationship between the properties of liquid and solid water, so it is a law.
• This is a possible explanation for the origin of birds, so it is a hypothesis.
• This is a statement that tries to explain the relationship between the temperature and the density of air based on fundamental principles, so it is a theory.
• The temperature is measured before and after a change is made in a system, so these are observations.
• This is an analysis designed to test a hypothesis (in this case, the manufacturer’s claim of purity), so it is an experiment.

Exercise \(\PageIndex{1}\) 

Classify each statement as a law, a theory, an experiment, a hypothesis, a qualitative observation, or a quantitative observation.

• Measured amounts of acid were added to a Rolaids tablet to see whether it really “consumes 47 times its weight in excess stomach acid.”
• Heat always flows from hot objects to cooler ones, not in the opposite direction.
• The universe was formed by a massive explosion that propelled matter into a vacuum.
• Michael Jordan is the greatest pure shooter to ever play professional basketball.
• Limestone is relatively insoluble in water, but dissolves readily in dilute acid with the evolution of a gas.

The scientific method is a method of investigation involving experimentation and observation to acquire new knowledge, solve problems, and answer questions. The key steps in the scientific method include the following:

• Step 1: Make observations.
• Step 2: Formulate a hypothesis.
• Step 3: Test the hypothesis through experimentation.
• Step 4: Accept or modify the hypothesis.
• Step 5: Develop into a law and/or a theory.

High school biology

Course: high school biology   >   unit 1.

• Biology overview
• Preparing to study biology
• What is life?
• The scientific method
• Data to justify experimental claims examples
• Scientific method and data analysis
• Introduction to experimental design
• Controlled experiments

Biology and the scientific method review

• Experimental design and bias

The nature of biology

Properties of life.

• Organization: Living things are highly organized (meaning they contain specialized, coordinated parts) and are made up of one or more cells .
• Metabolism: Living things must use energy and consume nutrients to carry out the chemical reactions that sustain life. The sum total of the biochemical reactions occurring in an organism is called its metabolism .
• Homeostasis : Living organisms regulate their internal environment to maintain the relatively narrow range of conditions needed for cell function.
• Growth : Living organisms undergo regulated growth. Individual cells become larger in size, and multicellular organisms accumulate many cells through cell division.
• Reproduction : Living organisms can reproduce themselves to create new organisms.
• Response : Living organisms respond to stimuli or changes in their environment.
• Evolution : Populations of living organisms can undergo evolution , meaning that the genetic makeup of a population may change over time.

Scientific methodology

Scientific method example: failure to toast.

• Observation: the toaster won't toast.
• Question: Why won't my toaster toast?
• Hypothesis: Maybe the outlet is broken.
• Prediction: If I plug the toaster into a different outlet, then it will toast the bread.
• Test of prediction: Plug the toaster into a different outlet and try again.
• Iteration time!

Experimental design

Reducing errors and bias.

• Having a large sample size in the experiment: This helps to account for any small differences among the test subjects that may provide unexpected results.
• Repeating experimental trials multiple times: Errors may result from slight differences in test subjects, or mistakes in methodology or data collection. Repeating trials helps reduce those effects.
• Including all data points: Sometimes it is tempting to throw away data points that are inconsistent with the proposed hypothesis. However, this makes for an inaccurate study! All data points need to be included, whether they support the hypothesis or not.
• Using placebos , when appropriate: Placebos prevent the test subjects from knowing whether they received a real therapeutic substance. This helps researchers determine whether a substance has a true effect.
• Implementing double-blind studies , when appropriate: Double-blind studies prevent researchers from knowing the status of a particular participant. This helps eliminate observer bias.

Communicating findings

Things to remember.

• A hypothesis is not necessarily the right explanation. Instead, it is a possible explanation that can be tested to see if it is likely correct, or if a new hypothesis needs to be made.
• Not all explanations can be considered a hypothesis. A hypothesis must be testable and falsifiable in order to be valid. For example, “The universe is beautiful" is not a good hypothesis, because there is no experiment that could test this statement and show it to be false.
• In most cases, the scientific method is an iterative process. In other words, it's a cycle rather than a straight line. The result of one experiment often becomes feedback that raises questions for more experimentation.
• Scientists use the word "theory" in a very different way than non-scientists. When many people say "I have a theory," they really mean "I have a guess." Scientific theories, on the other hand, are well-tested and highly reliable scientific explanations of natural phenomena. They unify many repeated observations and data collected from lots of experiments.

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Conclusions and Scientific Explanations

Review of the types of investigations.

Each of the three types of investigations involve different parts of the scientific inquiry process. The chart below reviews the parts of scientific inquiry in each type of investigation.

Notice that in all three types of investigations, conclusions are made.

Formulating Reasonable Explanations and Valid Conclusions

Data collected during an experiment and its repetitions are used to develop a conclusion. Data can either support the hypothesis or not support it. If your experimental results do not match your hypothesis, this does not mean your hypothesis is wrong. Perhaps you could redesign your experiment, or find sources of error.

When asked to identify conclusions based on investigation descriptions, data tables, graphs and drawings, remember to do the following:

• Look back at the hypothesis.
• Identify the independent and dependent variables.
• Look at the data carefully.
• Look for an overall trend in the data.
• Determine if the data support or don't support the hypothesis.
• Write a statement that sums up the results and reflects on the hypothesis, or if answering a multiple choice question, go through each statement and determine if the data supports the statement and if the statement is relevant to the investigation.
• Determine if the data is reproducible and if the sampling is done randomly.

Note that a good conclusion does not usually contain words such as best, worst, always, never. These words do not reflect on a hypothesis, are not measurable, and often connect to an opinion. Conclusions should be based on the data collected during the investigation and should be opinion and inference free.

Let's try making a valid conclusion together.

Read the description of the investigation and then analyze the graph.

A student conducted a comparative investigation to see which fertilizer would have the greatest effect on the growth of a plant. She grew 10 plants of the same type in the same type and size of planter. She watered the plants the same amount, and each plant received the same amount of sunlight. She gave each plant the same amount of fertilizer, but she gave 5 of the plants Fertilizer A and the other 5 plants Fertilizer B. She measured the growth of the plants each week. Displayed in the graph below is the average growth of the plants each week.

Practice: Drawing Conclusions

Now, it is your turn to practice drawing conclusions.

Copy and paste the link code above.

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