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How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Published by Nicolas at January 16th, 2024 , Revised On January 23, 2024

How To Write A Hypotheses – Guide For Students

The word “hypothesis” might conjure up images of scientists in white coats, but crafting a solid hypothesis is a crucial skill for students in any field. Whether you are analyzing Shakespeare’s sonnets or conducting a science experiment, a well-defined research hypothesis sets the stage for your dissertation or thesis and fuels your investigation. 

Table of Contents

Writing a hypothesis is a crucial step in the research process. A hypothesis serves as the foundation of your research paper because it guides the direction of your study and provides a clear framework for investigation. But how to write a hypothesis? This blog will help you craft one. Let’s get started.

What Is A Hypothesis

A hypothesis is a clear and testable thesis statement or prediction that serves as the foundation of a research study. It is formulated based on existing knowledge, observations, and theoretical frameworks. 

A hypothesis articulates the researcher’s expectations regarding the relationship between variables in a study.

Hypothesis Example

Students exposed to multimedia-enhanced teaching methods will demonstrate higher retention of information compared to those taught using traditional methods.

The formulation of a hypothesis is crucial for guiding the research process and providing a clear direction for data collection and analysis. A well-crafted research hypothesis not only makes the research purpose explicit but also sets the stage for drawing meaningful conclusions from the study’s findings.

What Is A Null Hypothesis And Alternative Hypothesis

There are two main types of hypotheses: the null hypothesis (H0) and the alternative hypothesis (H1 or Ha). 

The null hypothesis posits that there is no significant effect or relationship, while the alternative hypothesis suggests the presence of a significant effect or relationship.

For example, in a study investigating the effect of a new drug on blood pressure, the null hypothesis might state that there is no difference in blood pressure between the control group (not receiving the drug) and the experimental group (receiving the drug). The alternative hypothesis, on the other hand, would propose that there is a significant difference in blood pressure between the two groups.

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How To Write A Good Research Hypothesis

Writing a hypothesis involves a systematic process that guides your research and provides a clear and testable statement about the expected relationship between variables. Go through the MLA vs. APA guidelines before writing. Here are the steps to help you how to write a hypothesis:

Step 1: Identify The Research Topic

Clearly define the research topic or question that you want to investigate. Ensure that your research question is specific and focused, providing a clear direction for your study.

Step 2: Conduct A Literature Review

Review existing literature related to your research topic. A thorough literature review helps you understand what is already known in the field, identify gaps, and build a foundation for formulating your hypothesis.

Step 3: Define Variables

Identify the variables involved in your study. The independent variable is the factor you manipulate, and the dependent variable is the one you measure. Clearly define the characteristics or conditions you are studying.

Step 4: Establish The Relationship

Determine the expected relationship between the independent and dependent variables. Will a change in the independent variable lead to a change in the dependent variable? Specify whether you anticipate a positive, negative, or no relationship.

Step 5: Formulate The Null Hypothesis (H0)

The null hypothesis represents the default position, suggesting that there is no significant effect or relationship between the variables you are studying. It serves as the baseline to be tested against. The null hypothesis is often denoted as H0.

Step 6: Formulate The Alternative Hypothesis (H1 or Ha)

The alternative hypothesis articulates the researcher’s expectation about the existence of a significant effect or relationship. It is what you aim to support with your research paper . The alternative hypothesis is denoted as H1 or Ha.

For example, if your research topic is about the effect of a new fertilizer on plant growth:

• Null Hypothesis (H0): There is no significant difference in plant growth between plants treated with the traditional fertilizer and those treated with the new fertilizer.
• Alternative Hypothesis (H1): There is a significant difference in plant growth between plants treated with the traditional fertilizer and those treated with the new fertilizer.

Step 7: Ensure Testability And Specificity

Confirm that your research hypothesis is testable and can be empirically investigated. Ensure that it is specific, providing a clear and measurable statement that can be validated or refuted through data collection and analysis.

Hypothesis Examples

What makes a good hypothesis.

• Clear Statement: A hypothesis should be stated clearly and precisely. It should be easily understandable and convey the expected relationship between variables.
• Testability: A hypothesis must be testable through empirical observation or experimentation. This means that there should be a feasible way to collect data and assess whether the expected relationship holds true.
• Specificity: The research hypothesis should be specific in terms of the variables involved and the nature of the expected relationship. Vague or ambiguous hypotheses can lead to unclear research outcomes.
• Measurability: Variables in a hypothesis should be measurable, meaning they can be quantified or observed objectively. This ensures that the research can be conducted with precision.
• Falsifiability: A good research hypothesis should be falsifiable, meaning there should be a possibility of proving it wrong. This concept is fundamental to the scientific method, as hypotheses that cannot be tested or disproven lack scientific validity.

Frequently Asked Questions

How to write a hypothesis.

• Clearly state the research question.
• Identify the variables involved.
• Formulate a clear and testable prediction.
• Use specific and measurable terms.
• Align the hypothesis with the research question.
• Distinguish between the null hypothesis (no effect) and alternative hypothesis (expected effect).
• Ensure the hypothesis is falsifiable and subject to empirical testing.

How to write a hypothesis for a lab?

• Identify the purpose of the lab.
• Clearly state the relationship between variables.
• Use concise language and specific terms.
• Make the hypothesis testable through experimentation.
• Align with the lab’s objectives.
• Include an if-then statement to express the expected outcome.
• Ensure clarity and relevance to the experimental setup.

What Is A Null Hypothesis?

A null hypothesis is a statement suggesting no effect or relationship between variables in a research study. It serves as the default assumption, stating that any observed differences or effects are due to chance. Researchers aim to reject the null hypothesis based on statistical evidence to support their alternative hypothesis.

How to write a null hypothesis?

• State there is no effect, difference, or relationship between variables.
• Use clear and specific language.
• Frame it in a testable manner.
• Align with the research question.
• Specify parameters for statistical testing.
• Consider it as the default assumption to be tested and potentially rejected in favour of the alternative hypothesis.

What is the p-value of a hypothesis test?

The p-value in a hypothesis test represents the probability of obtaining observed results, or more extreme ones, if the null hypothesis is true. A lower p-value suggests stronger evidence against the null hypothesis, often leading to its rejection. Common significance thresholds include 0.05 or 0.01.

How to write a hypothesis in science?

• Clearly state the research question
• Identify the variables and their relationship.
• Formulate a testable and falsifiable prediction.
• Use specific, measurable terms.
• Distinguish between the null and alternative hypotheses.
• Ensure clarity and relevance to the scientific investigation.

How to write a hypothesis for a research proposal?

• Clearly define the research question.
• Identify variables and their expected relationship.
• Formulate a specific, testable hypothesis.
• Align the hypothesis with the proposal’s objectives.
• Clearly articulate the null hypothesis.
• Use concise language and measurable terms.
• Ensure the hypothesis aligns with the proposed research methodology.

How to write a good hypothesis psychology?

• Formulate a specific and testable prediction.
• Use precise and measurable terms.
• Align the hypothesis with psychological theories.
• Articulate the null hypothesis.
• Ensure the hypothesis guides empirical testing in psychological research.

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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The Craft of Writing a Strong Hypothesis

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

• Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
• Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

• A research hypothesis has to be simple yet clear to look justifiable enough.
• It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
• It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
• A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
• If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
• A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

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How to Write a Hypothesis: A Step-by-Step Guide

Introduction

An overview of the research hypothesis, different types of hypotheses, variables in a hypothesis, how to formulate an effective research hypothesis, designing a study around your hypothesis.

The scientific method can derive and test predictions as hypotheses. Empirical research can then provide support (or lack thereof) for the hypotheses. Even failure to find support for a hypothesis still represents a valuable contribution to scientific knowledge. Let's look more closely at the idea of the hypothesis and the role it plays in research.

As much as the term exists in everyday language, there is a detailed development that informs the word "hypothesis" when applied to research. A good research hypothesis is informed by prior research and guides research design and data analysis , so it is important to understand how a hypothesis is defined and understood by researchers.

What is the simple definition of a hypothesis?

A hypothesis is a testable prediction about an outcome between two or more variables . It functions as a navigational tool in the research process, directing what you aim to predict and how.

What is the hypothesis for in research?

In research, a hypothesis serves as the cornerstone for your empirical study. It not only lays out what you aim to investigate but also provides a structured approach for your data collection and analysis.

Essentially, it bridges the gap between the theoretical and the empirical, guiding your investigation throughout its course.

What is an example of a hypothesis?

If you are studying the relationship between physical exercise and mental health, a suitable hypothesis could be: "Regular physical exercise leads to improved mental well-being among adults."

This statement constitutes a specific and testable hypothesis that directly relates to the variables you are investigating.

What makes a good hypothesis?

A good hypothesis possesses several key characteristics. Firstly, it must be testable, allowing you to analyze data through empirical means, such as observation or experimentation, to assess if there is significant support for the hypothesis. Secondly, a hypothesis should be specific and unambiguous, giving a clear understanding of the expected relationship between variables. Lastly, it should be grounded in existing research or theoretical frameworks , ensuring its relevance and applicability.

Understanding the types of hypotheses can greatly enhance how you construct and work with hypotheses. While all hypotheses serve the essential function of guiding your study, there are varying purposes among the types of hypotheses. In addition, all hypotheses stand in contrast to the null hypothesis, or the assumption that there is no significant relationship between the variables .

Here, we explore various kinds of hypotheses to provide you with the tools needed to craft effective hypotheses for your specific research needs. Bear in mind that many of these hypothesis types may overlap with one another, and the specific type that is typically used will likely depend on the area of research and methodology you are following.

Null hypothesis

The null hypothesis is a statement that there is no effect or relationship between the variables being studied. In statistical terms, it serves as the default assumption that any observed differences are due to random chance.

For example, if you're studying the effect of a drug on blood pressure, the null hypothesis might state that the drug has no effect.

Alternative hypothesis

Contrary to the null hypothesis, the alternative hypothesis suggests that there is a significant relationship or effect between variables.

Using the drug example, the alternative hypothesis would posit that the drug does indeed affect blood pressure. This is what researchers aim to prove.

Simple hypothesis

A simple hypothesis makes a prediction about the relationship between two variables, and only two variables.

For example, "Increased study time results in better exam scores." Here, "study time" and "exam scores" are the only variables involved.

Complex hypothesis

A complex hypothesis, as the name suggests, involves more than two variables. For instance, "Increased study time and access to resources result in better exam scores." Here, "study time," "access to resources," and "exam scores" are all variables.

This hypothesis refers to multiple potential mediating variables. Other hypotheses could also include predictions about variables that moderate the relationship between the independent variable and dependent variable .

Directional hypothesis

A directional hypothesis specifies the direction of the expected relationship between variables. For example, "Eating more fruits and vegetables leads to a decrease in heart disease."

Here, the direction of heart disease is explicitly predicted to decrease, due to effects from eating more fruits and vegetables. All hypotheses typically specify the expected direction of the relationship between the independent and dependent variable, such that researchers can test if this prediction holds in their data analysis .

Statistical hypothesis

A statistical hypothesis is one that is testable through statistical methods, providing a numerical value that can be analyzed. This is commonly seen in quantitative research .

For example, "There is a statistically significant difference in test scores between students who study for one hour and those who study for two."

Empirical hypothesis

An empirical hypothesis is derived from observations and is tested through empirical methods, often through experimentation or survey data . Empirical hypotheses may also be assessed with statistical analyses.

For example, "Regular exercise is correlated with a lower incidence of depression," could be tested through surveys that measure exercise frequency and depression levels.

Causal hypothesis

A causal hypothesis proposes that one variable causes a change in another. This type of hypothesis is often tested through controlled experiments.

For example, "Smoking causes lung cancer," assumes a direct causal relationship.

Associative hypothesis

Unlike causal hypotheses, associative hypotheses suggest a relationship between variables but do not imply causation.

For instance, "People who smoke are more likely to get lung cancer," notes an association but doesn't claim that smoking causes lung cancer directly.

Relational hypothesis

A relational hypothesis explores the relationship between two or more variables but doesn't specify the nature of the relationship.

For example, "There is a relationship between diet and heart health," leaves the nature of the relationship (causal, associative, etc.) open to interpretation.

Logical hypothesis

A logical hypothesis is based on sound reasoning and logical principles. It's often used in theoretical research to explore abstract concepts, rather than being based on empirical data.

For example, "If all men are mortal and Socrates is a man, then Socrates is mortal," employs logical reasoning to make its point.

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In any research hypothesis, variables play a critical role. These are the elements or factors that the researcher manipulates, controls, or measures. Understanding variables is essential for crafting a clear, testable hypothesis and for the stages of research that follow, such as data collection and analysis.

In the realm of hypotheses, there are generally two types of variables to consider: independent and dependent. Independent variables are what you, as the researcher, manipulate or change in your study. It's considered the cause in the relationship you're investigating. For instance, in a study examining the impact of sleep duration on academic performance, the independent variable would be the amount of sleep participants get.

Conversely, the dependent variable is the outcome you measure to gauge the effect of your manipulation. It's the effect in the cause-and-effect relationship. The dependent variable thus refers to the main outcome of interest in your study. In the same sleep study example, the academic performance, perhaps measured by exam scores or GPA, would be the dependent variable.

Beyond these two primary types, you might also encounter control variables. These are variables that could potentially influence the outcome and are therefore kept constant to isolate the relationship between the independent and dependent variables . For example, in the sleep and academic performance study, control variables could include age, diet, or even the subject of study.

By clearly identifying and understanding the roles of these variables in your hypothesis, you set the stage for a methodologically sound research project. It helps you develop focused research questions, design appropriate experiments or observations, and carry out meaningful data analysis . It's a step that lays the groundwork for the success of your entire study.

Crafting a strong, testable hypothesis is crucial for the success of any research project. It sets the stage for everything from your study design to data collection and analysis . Below are some key considerations to keep in mind when formulating your hypothesis:

• Be specific : A vague hypothesis can lead to ambiguous results and interpretations . Clearly define your variables and the expected relationship between them.
• Ensure testability : A good hypothesis should be testable through empirical means, whether by observation , experimentation, or other forms of data analysis.
• Ground in literature : Before creating your hypothesis, consult existing research and theories. This not only helps you identify gaps in current knowledge but also gives you valuable context and credibility for crafting your hypothesis.
• Use simple language : While your hypothesis should be conceptually sound, it doesn't have to be complicated. Aim for clarity and simplicity in your wording.
• State direction, if applicable : If your hypothesis involves a directional outcome (e.g., "increase" or "decrease"), make sure to specify this. You also need to think about how you will measure whether or not the outcome moved in the direction you predicted.
• Keep it focused : One of the common pitfalls in hypothesis formulation is trying to answer too many questions at once. Keep your hypothesis focused on a specific issue or relationship.
• Account for control variables : Identify any variables that could potentially impact the outcome and consider how you will control for them in your study.
• Be ethical : Make sure your hypothesis and the methods for testing it comply with ethical standards , particularly if your research involves human or animal subjects.

Designing your study involves multiple key phases that help ensure the rigor and validity of your research. Here we discuss these crucial components in more detail.

Literature review

Starting with a comprehensive literature review is essential. This step allows you to understand the existing body of knowledge related to your hypothesis and helps you identify gaps that your research could fill. Your research should aim to contribute some novel understanding to existing literature, and your hypotheses can reflect this. A literature review also provides valuable insights into how similar research projects were executed, thereby helping you fine-tune your own approach.

Research methods

Choosing the right research methods is critical. Whether it's a survey, an experiment, or observational study, the methodology should be the most appropriate for testing your hypothesis. Your choice of methods will also depend on whether your research is quantitative, qualitative, or mixed-methods. Make sure the chosen methods align well with the variables you are studying and the type of data you need.

Preliminary research

Before diving into a full-scale study, it’s often beneficial to conduct preliminary research or a pilot study . This allows you to test your research methods on a smaller scale, refine your tools, and identify any potential issues. For instance, a pilot survey can help you determine if your questions are clear and if the survey effectively captures the data you need. This step can save you both time and resources in the long run.

Data analysis

Finally, planning your data analysis in advance is crucial for a successful study. Decide which statistical or analytical tools are most suited for your data type and research questions . For quantitative research, you might opt for t-tests, ANOVA, or regression analyses. For qualitative research , thematic analysis or grounded theory may be more appropriate. This phase is integral for interpreting your results and drawing meaningful conclusions in relation to your research question.

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How To Write a Strong Research Hypothesis

Are you looking to take your research project to the next level? Have you heard of the power of a hypothesis but need to figure out how to formulate one that will unlock potential discoveries? We can help!

So get ready; it's time to dive into unlocking the power of research! This blog post will explore what makes a well-crafted and powerful hypothesis - from identifying a research question to developing supporting evidence.

By learning how to craft a compelling hypothesis, you'll have more tremendous success in every step of your research project.

What are hypotheses, and why are they important?

A hypothesis is an educated guess or a proposition based on limited evidence as a starting point for further investigation. It provides a framework for research and allows researchers to refine their ideas, collect data, and draw conclusions. Hypotheses are essential to the process because they will enable us to organize our thoughts and test theories properly.

Hypotheses are used in many fields , from medicine to psychology to economics. In each area, developing hypotheses based on observations enable researchers to make predictions about their data and guide them toward finding meaningful results.

For example, in medicine, hypotheses can be used to predict which treatments will be most effective for particular conditions or which drugs may have adverse effects when taken together. This allows doctors to make better decisions when caring for patients.

In psychology, hypotheses are often used in experiments to determine whether certain variables influence behavior or mental processes. By testing different combinations of variables, psychologists can identify patterns and understand why people behave the way they do.

In economics, hypotheses provide economists with a framework for analyzing the relationship between economic variables such as wages and consumer spending habits. By understanding these relationships, economists can better understand how economic forces affect the economy.

Overall, hypotheses play an essential role in helping scientists develop new ideas and draw meaningful conclusions from the collected data. Without taking the step to create hypotheses, it would be difficult for researchers to make sense of the vast amounts of information available today and use it effectively in their investigations.

How to determine an effective research question to form your hypothesis

When conducting research, having a compelling research question is critical . Properly formulating this question will allow the researcher to develop their hypothesis. A research question provides a clear and focused goal for your research study and also gives direction on how to get there. A compelling research question should be specific, answerable in the context of your field of study, significant, novel (not already answered by previous studies), and timely – that is, relevant to current events or trends.

Before determining the best research question, you must first understand your topic. Think about the area of knowledge that interests you most and narrow it down to a single theme or concept within this topic. Focus on what interests you most within this theme, and make sure there is room for further exploration and analysis. Once you have chosen a specific topic and narrowed down your focus, you can begin formulating questions related to your project.

To ensure relevance and impact to your field of study, choose questions that address essential issues in the literature or suggest solutions to existing problems. Avoid overly broad topics with unclear objectives; instead, opt for focused questions to enable targeted data collection and analysis with concrete results.

Additionally, consider time frames when formulating questions. If the issue has been discussed extensively in the past but has not been revisited recently, then it's likely not worthy of a new investigation.

Once you have developed some potential questions related to your topic, review them carefully and decide which question best captures the essence of what you want to learn through researching this topic.

Ask yourself:

• Is this question answerable?
• Does it fit within my field of study?
• Is it significant enough?
• Would its findings be novel?

If so, then congratulations! You have identified a compelling research question.

Tips for crafting a well-crafted hypothesis

Once you have formulated the official research question, you may develop the formal hypothesis. When composing a hypothesis, it's essential to think carefully about the question you are trying to answer.

A solid hypothesis should be testable, meaning that it can be verified or disproved through research. It should also be specific and focused on one issue at a time. Here are some tips for crafting a well-crafted hypothesis:

• Consider the goal of your research: Think about what it is that you want to learn or determine from your experiment and make sure that your hypothesis reflects this goal.
• Create an educated guess as to why something is happening: Your hypothesis should explain why something is occurring based on what evidence you already have and direct further investigation into the matter. For example, if you hypothesize that increased carbon dioxide levels in the atmosphere will lead to global warming, your research should focus on examining this relationship further.
• Define any variables or parameters involved in the experiment: This includes things like temperature or chemical composition that could potentially affect the outcome of any experiments done in pursuit of testing your hypothesis.
• Use clear and precise language: Make sure your hypothesis is written with clear and precise language so that anyone reading it can understand exactly what you are attempting to investigate or explain. Avoid complex words and keep sentences short whenever possible.

Following these simple tips will help ensure that your hypothesis is well-crafted and ready for testing!

Examples of evidence that can support your hypothesis

When it comes to developing a hypothesis, supporting evidence is essential for making sure it holds up. This evidence helps strengthen the argument that is being driven by providing facts and logical reasoning that support the hypothesis.

Examples of evidence that can be used to back up a hypothesis include using data from experiments, case studies, and other research projects. Data from experiments can provide insight into how certain variables interact to form a particular outcome.

Case studies may offer greater depth in understanding a specific phenomenon's cause and effect; research projects may yield results that confirm or refute existing theories on a subject.

In addition to these traditional forms of evidence, personal experiences or observations can also help to support a hypothesis. For example, if someone's daily commute has been consistently faster since they changed routes, they could use their personal experience to argue that making this change resulted in shorter commutes.

Similarly, suppose someone has witnessed how two variables consistently coincide (i.e., when one goes up, another goes down). In that case, this could be used to support the notion that there is some correlation between these two aspects.

Overall, evidence to support your hypothesis is crucial for ensuring its validity and credibility. While conducting experiments or researching may seem like time-consuming processes, having solid supporting evidence will make it much easier to defend your ideas convincingly when challenged.

Therefore, it is crucial to take the time necessary to gather credible sources of information to provide the most substantial possible backing for your hypotheses.

Understanding the potential of hypotheses and how they can help your research project progress

The power of research lies in the ability to develop and test hypotheses. A hypothesis is a statement or an idea that can be tested to determine its validity.

Essentially, it is a form of educated guesswork that helps researchers form conclusions about their data. By developing a hypothesis for a research project, you are effectively setting up the framework for further exploration.

When developing a hypothesis, you must consider both the expected outcomes and possible alternative explanations. This will help you focus on testing the possible results without getting sidetracked by irrelevant information. Once you have established a concrete hypothesis, it can then be used as a basis for further research and experimentation.

The process of testing hypotheses is an integral part of the scientific method and can help researchers build confidence in their findings and conclusions. Through careful observation and experimentation, researchers can compare their results against what they initially hypothesized, allowing them to draw more accurate conclusions about their data. As such, hypotheses play an essential role in helping researchers connect the dots between different pieces of evidence and form meaningful conclusions.

Overall, understanding how hypotheses can be used in research projects can be immensely beneficial in helping progress towards reaching meaningful insights from their data. By setting up expectations ahead of time and then testing them against real-world conditions, researchers can gain valuable insights that could potentially change the way we understand our world – now that's something worth exploring!

Final thoughts

A hypothesis is a proposed explanation for an observable phenomenon. It's important to note that hypotheses are not the same thing as theories–a theory is a much broader and well-established frame of reference that explains multiple phenomena.

Generally, scientists form a research question and then narrow it down to a testable hypothesis. After making observations and conducting experiments to gather data, researchers can use evidence to support or reject the hypothesis.

By following these steps to formulate a solid hypothesis, you will be on your way to developing a successful research project. Happy researching!

Header image by Bnenin .

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How to Develop a Good Research Hypothesis

The story of a research study begins by asking a question. Researchers all around the globe are asking curious questions and formulating research hypothesis. However, whether the research study provides an effective conclusion depends on how well one develops a good research hypothesis. Research hypothesis examples could help researchers get an idea as to how to write a good research hypothesis.

This blog will help you understand what is a research hypothesis, its characteristics and, how to formulate a research hypothesis

Table of Contents

What is Hypothesis?

Hypothesis is an assumption or an idea proposed for the sake of argument so that it can be tested. It is a precise, testable statement of what the researchers predict will be outcome of the study.  Hypothesis usually involves proposing a relationship between two variables: the independent variable (what the researchers change) and the dependent variable (what the research measures).

What is a Research Hypothesis?

Research hypothesis is a statement that introduces a research question and proposes an expected result. It is an integral part of the scientific method that forms the basis of scientific experiments. Therefore, you need to be careful and thorough when building your research hypothesis. A minor flaw in the construction of your hypothesis could have an adverse effect on your experiment. In research, there is a convention that the hypothesis is written in two forms, the null hypothesis, and the alternative hypothesis (called the experimental hypothesis when the method of investigation is an experiment).

Characteristics of a Good Research Hypothesis

As the hypothesis is specific, there is a testable prediction about what you expect to happen in a study. You may consider drawing hypothesis from previously published research based on the theory.

A good research hypothesis involves more effort than just a guess. In particular, your hypothesis may begin with a question that could be further explored through background research.

To help you formulate a promising research hypothesis, you should ask yourself the following questions:

• Is the language clear and focused?
• What is the relationship between your hypothesis and your research topic?
• Is your hypothesis testable? If yes, then how?
• What are the possible explanations that you might want to explore?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate your variables without hampering the ethical standards?
• Does your research predict the relationship and outcome?
• Is your research simple and concise (avoids wordiness)?
• Is it clear with no ambiguity or assumptions about the readers’ knowledge
• Is your research observable and testable results?
• Is it relevant and specific to the research question or problem?

The questions listed above can be used as a checklist to make sure your hypothesis is based on a solid foundation. Furthermore, it can help you identify weaknesses in your hypothesis and revise it if necessary.

Source: Educational Hub

How to formulate a research hypothesis.

A testable hypothesis is not a simple statement. It is rather an intricate statement that needs to offer a clear introduction to a scientific experiment, its intentions, and the possible outcomes. However, there are some important things to consider when building a compelling hypothesis.

1. State the problem that you are trying to solve.

Make sure that the hypothesis clearly defines the topic and the focus of the experiment.

2. Try to write the hypothesis as an if-then statement.

Follow this template: If a specific action is taken, then a certain outcome is expected.

3. Define the variables

Independent variables are the ones that are manipulated, controlled, or changed. Independent variables are isolated from other factors of the study.

Dependent variables , as the name suggests are dependent on other factors of the study. They are influenced by the change in independent variable.

4. Scrutinize the hypothesis

Evaluate assumptions, predictions, and evidence rigorously to refine your understanding.

Types of Research Hypothesis

The types of research hypothesis are stated below:

1. Simple Hypothesis

It predicts the relationship between a single dependent variable and a single independent variable.

2. Complex Hypothesis

It predicts the relationship between two or more independent and dependent variables.

3. Directional Hypothesis

It specifies the expected direction to be followed to determine the relationship between variables and is derived from theory. Furthermore, it implies the researcher’s intellectual commitment to a particular outcome.

4. Non-directional Hypothesis

It does not predict the exact direction or nature of the relationship between the two variables. The non-directional hypothesis is used when there is no theory involved or when findings contradict previous research.

5. Associative and Causal Hypothesis

The associative hypothesis defines interdependency between variables. A change in one variable results in the change of the other variable. On the other hand, the causal hypothesis proposes an effect on the dependent due to manipulation of the independent variable.

6. Null Hypothesis

Null hypothesis states a negative statement to support the researcher’s findings that there is no relationship between two variables. There will be no changes in the dependent variable due the manipulation of the independent variable. Furthermore, it states results are due to chance and are not significant in terms of supporting the idea being investigated.

7. Alternative Hypothesis

It states that there is a relationship between the two variables of the study and that the results are significant to the research topic. An experimental hypothesis predicts what changes will take place in the dependent variable when the independent variable is manipulated. Also, it states that the results are not due to chance and that they are significant in terms of supporting the theory being investigated.

Research Hypothesis Examples of Independent and Dependent Variables

Research Hypothesis Example 1 The greater number of coal plants in a region (independent variable) increases water pollution (dependent variable). If you change the independent variable (building more coal factories), it will change the dependent variable (amount of water pollution).

Research Hypothesis Example 2 What is the effect of diet or regular soda (independent variable) on blood sugar levels (dependent variable)? If you change the independent variable (the type of soda you consume), it will change the dependent variable (blood sugar levels)

You should not ignore the importance of the above steps. The validity of your experiment and its results rely on a robust testable hypothesis. Developing a strong testable hypothesis has few advantages, it compels us to think intensely and specifically about the outcomes of a study. Consequently, it enables us to understand the implication of the question and the different variables involved in the study. Furthermore, it helps us to make precise predictions based on prior research. Hence, forming a hypothesis would be of great value to the research. Here are some good examples of testable hypotheses.

More importantly, you need to build a robust testable research hypothesis for your scientific experiments. A testable hypothesis is a hypothesis that can be proved or disproved as a result of experimentation.

Importance of a Testable Hypothesis

To devise and perform an experiment using scientific method, you need to make sure that your hypothesis is testable. To be considered testable, some essential criteria must be met:

• There must be a possibility to prove that the hypothesis is true.
• There must be a possibility to prove that the hypothesis is false.
• The results of the hypothesis must be reproducible.

Without these criteria, the hypothesis and the results will be vague. As a result, the experiment will not prove or disprove anything significant.

What are your experiences with building hypotheses for scientific experiments? What challenges did you face? How did you overcome these challenges? Please share your thoughts with us in the comments section.

Frequently Asked Questions

The steps to write a research hypothesis are: 1. Stating the problem: Ensure that the hypothesis defines the research problem 2. Writing a hypothesis as an 'if-then' statement: Include the action and the expected outcome of your study by following a ‘if-then’ structure. 3. Defining the variables: Define the variables as Dependent or Independent based on their dependency to other factors. 4. Scrutinizing the hypothesis: Identify the type of your hypothesis

Hypothesis testing is a statistical tool which is used to make inferences about a population data to draw conclusions for a particular hypothesis.

Hypothesis in statistics is a formal statement about the nature of a population within a structured framework of a statistical model. It is used to test an existing hypothesis by studying a population.

Research hypothesis is a statement that introduces a research question and proposes an expected result. It forms the basis of scientific experiments.

The different types of hypothesis in research are: • Null hypothesis: Null hypothesis is a negative statement to support the researcher’s findings that there is no relationship between two variables. • Alternate hypothesis: Alternate hypothesis predicts the relationship between the two variables of the study. • Directional hypothesis: Directional hypothesis specifies the expected direction to be followed to determine the relationship between variables. • Non-directional hypothesis: Non-directional hypothesis does not predict the exact direction or nature of the relationship between the two variables. • Simple hypothesis: Simple hypothesis predicts the relationship between a single dependent variable and a single independent variable. • Complex hypothesis: Complex hypothesis predicts the relationship between two or more independent and dependent variables. • Associative and casual hypothesis: Associative and casual hypothesis predicts the relationship between two or more independent and dependent variables. • Empirical hypothesis: Empirical hypothesis can be tested via experiments and observation. • Statistical hypothesis: A statistical hypothesis utilizes statistical models to draw conclusions about broader populations.

Wow! You really simplified your explanation that even dummies would find it easy to comprehend. Thank you so much.

Thanks a lot for your valuable guidance.

I enjoy reading the post. Hypotheses are actually an intrinsic part in a study. It bridges the research question and the methodology of the study.

Useful piece!

This is awesome.Wow.

It very interesting to read the topic, can you guide me any specific example of hypothesis process establish throw the Demand and supply of the specific product in market

Nicely explained

It is really a useful for me Kindly give some examples of hypothesis

It was a well explained content ,can you please give me an example with the null and alternative hypothesis illustrated

clear and concise. thanks.

So Good so Amazing

Good to learn

Thanks a lot for explaining to my level of understanding

Explained well and in simple terms. Quick read! Thank you

It awesome. It has really positioned me in my research project

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

• Manuscript Preparation

What is and How to Write a Good Hypothesis in Research?

• 4 minute read

Table of Contents

One of the most important aspects of conducting research is constructing a strong hypothesis. But what makes a hypothesis in research effective? In this article, we’ll look at the difference between a hypothesis and a research question, as well as the elements of a good hypothesis in research. We’ll also include some examples of effective hypotheses, and what pitfalls to avoid.

What is a Hypothesis in Research?

Simply put, a hypothesis is a research question that also includes the predicted or expected result of the research. Without a hypothesis, there can be no basis for a scientific or research experiment. As such, it is critical that you carefully construct your hypothesis by being deliberate and thorough, even before you set pen to paper. Unless your hypothesis is clearly and carefully constructed, any flaw can have an adverse, and even grave, effect on the quality of your experiment and its subsequent results.

Research Question vs Hypothesis

It’s easy to confuse research questions with hypotheses, and vice versa. While they’re both critical to the Scientific Method, they have very specific differences. Primarily, a research question, just like a hypothesis, is focused and concise. But a hypothesis includes a prediction based on the proposed research, and is designed to forecast the relationship of and between two (or more) variables. Research questions are open-ended, and invite debate and discussion, while hypotheses are closed, e.g. “The relationship between A and B will be C.”

A hypothesis is generally used if your research topic is fairly well established, and you are relatively certain about the relationship between the variables that will be presented in your research. Since a hypothesis is ideally suited for experimental studies, it will, by its very existence, affect the design of your experiment. The research question is typically used for new topics that have not yet been researched extensively. Here, the relationship between different variables is less known. There is no prediction made, but there may be variables explored. The research question can be casual in nature, simply trying to understand if a relationship even exists, descriptive or comparative.

How to Write Hypothesis in Research

Writing an effective hypothesis starts before you even begin to type. Like any task, preparation is key, so you start first by conducting research yourself, and reading all you can about the topic that you plan to research. From there, you’ll gain the knowledge you need to understand where your focus within the topic will lie.

Remember that a hypothesis is a prediction of the relationship that exists between two or more variables. Your job is to write a hypothesis, and design the research, to “prove” whether or not your prediction is correct. A common pitfall is to use judgments that are subjective and inappropriate for the construction of a hypothesis. It’s important to keep the focus and language of your hypothesis objective.

An effective hypothesis in research is clearly and concisely written, and any terms or definitions clarified and defined. Specific language must also be used to avoid any generalities or assumptions.

Use the following points as a checklist to evaluate the effectiveness of your research hypothesis:

• Predicts the relationship and outcome
• Simple and concise – avoid wordiness
• Clear with no ambiguity or assumptions about the readers’ knowledge
• Observable and testable results
• Relevant and specific to the research question or problem

Research Hypothesis Example

Perhaps the best way to evaluate whether or not your hypothesis is effective is to compare it to those of your colleagues in the field. There is no need to reinvent the wheel when it comes to writing a powerful research hypothesis. As you’re reading and preparing your hypothesis, you’ll also read other hypotheses. These can help guide you on what works, and what doesn’t, when it comes to writing a strong research hypothesis.

Here are a few generic examples to get you started.

Eating an apple each day, after the age of 60, will result in a reduction of frequency of physician visits.

Budget airlines are more likely to receive more customer complaints. A budget airline is defined as an airline that offers lower fares and fewer amenities than a traditional full-service airline. (Note that the term “budget airline” is included in the hypothesis.

Workplaces that offer flexible working hours report higher levels of employee job satisfaction than workplaces with fixed hours.

Each of the above examples are specific, observable and measurable, and the statement of prediction can be verified or shown to be false by utilizing standard experimental practices. It should be noted, however, that often your hypothesis will change as your research progresses.

Language Editing Plus

Elsevier’s Language Editing Plus service can help ensure that your research hypothesis is well-designed, and articulates your research and conclusions. Our most comprehensive editing package, you can count on a thorough language review by native-English speakers who are PhDs or PhD candidates. We’ll check for effective logic and flow of your manuscript, as well as document formatting for your chosen journal, reference checks, and much more.

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• v.36(50); 2021 Dec 27

Formulating Hypotheses for Different Study Designs

Durga prasanna misra.

1 Department of Clinical Immunology and Rheumatology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.

Armen Yuri Gasparyan

2 Departments of Rheumatology and Research and Development, Dudley Group NHS Foundation Trust (Teaching Trust of the University of Birmingham, UK), Russells Hall Hospital, Dudley, UK.

Olena Zimba

3 Department of Internal Medicine #2, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.

Marlen Yessirkepov

4 Department of Biology and Biochemistry, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

Vikas Agarwal

George d. kitas.

5 Centre for Epidemiology versus Arthritis, University of Manchester, Manchester, UK.

Generating a testable working hypothesis is the first step towards conducting original research. Such research may prove or disprove the proposed hypothesis. Case reports, case series, online surveys and other observational studies, clinical trials, and narrative reviews help to generate hypotheses. Observational and interventional studies help to test hypotheses. A good hypothesis is usually based on previous evidence-based reports. Hypotheses without evidence-based justification and a priori ideas are not received favourably by the scientific community. Original research to test a hypothesis should be carefully planned to ensure appropriate methodology and adequate statistical power. While hypotheses can challenge conventional thinking and may be controversial, they should not be destructive. A hypothesis should be tested by ethically sound experiments with meaningful ethical and clinical implications. The coronavirus disease 2019 pandemic has brought into sharp focus numerous hypotheses, some of which were proven (e.g. effectiveness of corticosteroids in those with hypoxia) while others were disproven (e.g. ineffectiveness of hydroxychloroquine and ivermectin).

Graphical Abstract

DEFINING WORKING AND STANDALONE SCIENTIFIC HYPOTHESES

Science is the systematized description of natural truths and facts. Routine observations of existing life phenomena lead to the creative thinking and generation of ideas about mechanisms of such phenomena and related human interventions. Such ideas presented in a structured format can be viewed as hypotheses. After generating a hypothesis, it is necessary to test it to prove its validity. Thus, hypothesis can be defined as a proposed mechanism of a naturally occurring event or a proposed outcome of an intervention. 1 , 2

Hypothesis testing requires choosing the most appropriate methodology and adequately powering statistically the study to be able to “prove” or “disprove” it within predetermined and widely accepted levels of certainty. This entails sample size calculation that often takes into account previously published observations and pilot studies. 2 , 3 In the era of digitization, hypothesis generation and testing may benefit from the availability of numerous platforms for data dissemination, social networking, and expert validation. Related expert evaluations may reveal strengths and limitations of proposed ideas at early stages of post-publication promotion, preventing the implementation of unsupported controversial points. 4

Thus, hypothesis generation is an important initial step in the research workflow, reflecting accumulating evidence and experts' stance. In this article, we overview the genesis and importance of scientific hypotheses and their relevance in the era of the coronavirus disease 2019 (COVID-19) pandemic.

DO WE NEED HYPOTHESES FOR ALL STUDY DESIGNS?

Broadly, research can be categorized as primary or secondary. In the context of medicine, primary research may include real-life observations of disease presentations and outcomes. Single case descriptions, which often lead to new ideas and hypotheses, serve as important starting points or justifications for case series and cohort studies. The importance of case descriptions is particularly evident in the context of the COVID-19 pandemic when unique, educational case reports have heralded a new era in clinical medicine. 5

Case series serve similar purpose to single case reports, but are based on a slightly larger quantum of information. Observational studies, including online surveys, describe the existing phenomena at a larger scale, often involving various control groups. Observational studies include variable-scale epidemiological investigations at different time points. Interventional studies detail the results of therapeutic interventions.

Secondary research is based on already published literature and does not directly involve human or animal subjects. Review articles are generated by secondary research. These could be systematic reviews which follow methods akin to primary research but with the unit of study being published papers rather than humans or animals. Systematic reviews have a rigid structure with a mandatory search strategy encompassing multiple databases, systematic screening of search results against pre-defined inclusion and exclusion criteria, critical appraisal of study quality and an optional component of collating results across studies quantitatively to derive summary estimates (meta-analysis). 6 Narrative reviews, on the other hand, have a more flexible structure. Systematic literature searches to minimise bias in selection of articles are highly recommended but not mandatory. 7 Narrative reviews are influenced by the authors' viewpoint who may preferentially analyse selected sets of articles. 8

In relation to primary research, case studies and case series are generally not driven by a working hypothesis. Rather, they serve as a basis to generate a hypothesis. Observational or interventional studies should have a hypothesis for choosing research design and sample size. The results of observational and interventional studies further lead to the generation of new hypotheses, testing of which forms the basis of future studies. Review articles, on the other hand, may not be hypothesis-driven, but form fertile ground to generate future hypotheses for evaluation. Fig. 1 summarizes which type of studies are hypothesis-driven and which lead on to hypothesis generation.

STANDARDS OF WORKING AND SCIENTIFIC HYPOTHESES

A review of the published literature did not enable the identification of clearly defined standards for working and scientific hypotheses. It is essential to distinguish influential versus not influential hypotheses, evidence-based hypotheses versus a priori statements and ideas, ethical versus unethical, or potentially harmful ideas. The following points are proposed for consideration while generating working and scientific hypotheses. 1 , 2 Table 1 summarizes these points.

Evidence-based data

A scientific hypothesis should have a sound basis on previously published literature as well as the scientist's observations. Randomly generated (a priori) hypotheses are unlikely to be proven. A thorough literature search should form the basis of a hypothesis based on published evidence. 7

Unless a scientific hypothesis can be tested, it can neither be proven nor be disproven. Therefore, a scientific hypothesis should be amenable to testing with the available technologies and the present understanding of science.

Supported by pilot studies

If a hypothesis is based purely on a novel observation by the scientist in question, it should be grounded on some preliminary studies to support it. For example, if a drug that targets a specific cell population is hypothesized to be useful in a particular disease setting, then there must be some preliminary evidence that the specific cell population plays a role in driving that disease process.

Testable by ethical studies

The hypothesis should be testable by experiments that are ethically acceptable. 9 For example, a hypothesis that parachutes reduce mortality from falls from an airplane cannot be tested using a randomized controlled trial. 10 This is because it is obvious that all those jumping from a flying plane without a parachute would likely die. Similarly, the hypothesis that smoking tobacco causes lung cancer cannot be tested by a clinical trial that makes people take up smoking (since there is considerable evidence for the health hazards associated with smoking). Instead, long-term observational studies comparing outcomes in those who smoke and those who do not, as was performed in the landmark epidemiological case control study by Doll and Hill, 11 are more ethical and practical.

Balance between scientific temper and controversy

Novel findings, including novel hypotheses, particularly those that challenge established norms, are bound to face resistance for their wider acceptance. Such resistance is inevitable until the time such findings are proven with appropriate scientific rigor. However, hypotheses that generate controversy are generally unwelcome. For example, at the time the pandemic of human immunodeficiency virus (HIV) and AIDS was taking foot, there were numerous deniers that refused to believe that HIV caused AIDS. 12 , 13 Similarly, at a time when climate change is causing catastrophic changes to weather patterns worldwide, denial that climate change is occurring and consequent attempts to block climate change are certainly unwelcome. 14 The denialism and misinformation during the COVID-19 pandemic, including unfortunate examples of vaccine hesitancy, are more recent examples of controversial hypotheses not backed by science. 15 , 16 An example of a controversial hypothesis that was a revolutionary scientific breakthrough was the hypothesis put forth by Warren and Marshall that Helicobacter pylori causes peptic ulcers. Initially, the hypothesis that a microorganism could cause gastritis and gastric ulcers faced immense resistance. When the scientists that proposed the hypothesis themselves ingested H. pylori to induce gastritis in themselves, only then could they convince the wider world about their hypothesis. Such was the impact of the hypothesis was that Barry Marshall and Robin Warren were awarded the Nobel Prize in Physiology or Medicine in 2005 for this discovery. 17 , 18

DISTINGUISHING THE MOST INFLUENTIAL HYPOTHESES

Influential hypotheses are those that have stood the test of time. An archetype of an influential hypothesis is that proposed by Edward Jenner in the eighteenth century that cowpox infection protects against smallpox. While this observation had been reported for nearly a century before this time, it had not been suitably tested and publicised until Jenner conducted his experiments on a young boy by demonstrating protection against smallpox after inoculation with cowpox. 19 These experiments were the basis for widespread smallpox immunization strategies worldwide in the 20th century which resulted in the elimination of smallpox as a human disease today. 20

Other influential hypotheses are those which have been read and cited widely. An example of this is the hygiene hypothesis proposing an inverse relationship between infections in early life and allergies or autoimmunity in adulthood. An analysis reported that this hypothesis had been cited more than 3,000 times on Scopus. 1

LESSONS LEARNED FROM HYPOTHESES AMIDST THE COVID-19 PANDEMIC

The COVID-19 pandemic devastated the world like no other in recent memory. During this period, various hypotheses emerged, understandably so considering the public health emergency situation with innumerable deaths and suffering for humanity. Within weeks of the first reports of COVID-19, aberrant immune system activation was identified as a key driver of organ dysfunction and mortality in this disease. 21 Consequently, numerous drugs that suppress the immune system or abrogate the activation of the immune system were hypothesized to have a role in COVID-19. 22 One of the earliest drugs hypothesized to have a benefit was hydroxychloroquine. Hydroxychloroquine was proposed to interfere with Toll-like receptor activation and consequently ameliorate the aberrant immune system activation leading to pathology in COVID-19. 22 The drug was also hypothesized to have a prophylactic role in preventing infection or disease severity in COVID-19. It was also touted as a wonder drug for the disease by many prominent international figures. However, later studies which were well-designed randomized controlled trials failed to demonstrate any benefit of hydroxychloroquine in COVID-19. 23 , 24 , 25 , 26 Subsequently, azithromycin 27 , 28 and ivermectin 29 were hypothesized as potential therapies for COVID-19, but were not supported by evidence from randomized controlled trials. The role of vitamin D in preventing disease severity was also proposed, but has not been proven definitively until now. 30 , 31 On the other hand, randomized controlled trials identified the evidence supporting dexamethasone 32 and interleukin-6 pathway blockade with tocilizumab as effective therapies for COVID-19 in specific situations such as at the onset of hypoxia. 33 , 34 Clues towards the apparent effectiveness of various drugs against severe acute respiratory syndrome coronavirus 2 in vitro but their ineffectiveness in vivo have recently been identified. Many of these drugs are weak, lipophilic bases and some others induce phospholipidosis which results in apparent in vitro effectiveness due to non-specific off-target effects that are not replicated inside living systems. 35 , 36

Another hypothesis proposed was the association of the routine policy of vaccination with Bacillus Calmette-Guerin (BCG) with lower deaths due to COVID-19. This hypothesis emerged in the middle of 2020 when COVID-19 was still taking foot in many parts of the world. 37 , 38 Subsequently, many countries which had lower deaths at that time point went on to have higher numbers of mortality, comparable to other areas of the world. Furthermore, the hypothesis that BCG vaccination reduced COVID-19 mortality was a classic example of ecological fallacy. Associations between population level events (ecological studies; in this case, BCG vaccination and COVID-19 mortality) cannot be directly extrapolated to the individual level. Furthermore, such associations cannot per se be attributed as causal in nature, and can only serve to generate hypotheses that need to be tested at the individual level. 39

IS TRADITIONAL PEER REVIEW EFFICIENT FOR EVALUATION OF WORKING AND SCIENTIFIC HYPOTHESES?

Traditionally, publication after peer review has been considered the gold standard before any new idea finds acceptability amongst the scientific community. Getting a work (including a working or scientific hypothesis) reviewed by experts in the field before experiments are conducted to prove or disprove it helps to refine the idea further as well as improve the experiments planned to test the hypothesis. 40 A route towards this has been the emergence of journals dedicated to publishing hypotheses such as the Central Asian Journal of Medical Hypotheses and Ethics. 41 Another means of publishing hypotheses is through registered research protocols detailing the background, hypothesis, and methodology of a particular study. If such protocols are published after peer review, then the journal commits to publishing the completed study irrespective of whether the study hypothesis is proven or disproven. 42 In the post-pandemic world, online research methods such as online surveys powered via social media channels such as Twitter and Instagram might serve as critical tools to generate as well as to preliminarily test the appropriateness of hypotheses for further evaluation. 43 , 44

Some radical hypotheses might be difficult to publish after traditional peer review. These hypotheses might only be acceptable by the scientific community after they are tested in research studies. Preprints might be a way to disseminate such controversial and ground-breaking hypotheses. 45 However, scientists might prefer to keep their hypotheses confidential for the fear of plagiarism of ideas, avoiding online posting and publishing until they have tested the hypotheses.

SUGGESTIONS ON GENERATING AND PUBLISHING HYPOTHESES

Publication of hypotheses is important, however, a balance is required between scientific temper and controversy. Journal editors and reviewers might keep in mind these specific points, summarized in Table 2 and detailed hereafter, while judging the merit of hypotheses for publication. Keeping in mind the ethical principle of primum non nocere, a hypothesis should be published only if it is testable in a manner that is ethically appropriate. 46 Such hypotheses should be grounded in reality and lend themselves to further testing to either prove or disprove them. It must be considered that subsequent experiments to prove or disprove a hypothesis have an equal chance of failing or succeeding, akin to tossing a coin. A pre-conceived belief that a hypothesis is unlikely to be proven correct should not form the basis of rejection of such a hypothesis for publication. In this context, hypotheses generated after a thorough literature search to identify knowledge gaps or based on concrete clinical observations on a considerable number of patients (as opposed to random observations on a few patients) are more likely to be acceptable for publication by peer-reviewed journals. Also, hypotheses should be considered for publication or rejection based on their implications for science at large rather than whether the subsequent experiments to test them end up with results in favour of or against the original hypothesis.

Hypotheses form an important part of the scientific literature. The COVID-19 pandemic has reiterated the importance and relevance of hypotheses for dealing with public health emergencies and highlighted the need for evidence-based and ethical hypotheses. A good hypothesis is testable in a relevant study design, backed by preliminary evidence, and has positive ethical and clinical implications. General medical journals might consider publishing hypotheses as a specific article type to enable more rapid advancement of science.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Data curation: Gasparyan AY, Misra DP, Zimba O, Yessirkepov M, Agarwal V, Kitas GD.

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Hypothesis Maker Online

Looking for a hypothesis maker? This online tool for students will help you formulate a beautiful hypothesis quickly, efficiently, and for free.

Are you looking for an effective hypothesis maker online? Worry no more; try our online tool for students and formulate your hypothesis within no time.

• 🔎 How to Use the Tool?
• ⚗️ What Is a Hypothesis in Science?

👍 What Does a Good Hypothesis Mean?

• 🧭 Steps to Making a Good Hypothesis

🔗 References

📄 hypothesis maker: how to use it.

Our hypothesis maker is a simple and efficient tool you can access online for free.

If you want to create a research hypothesis quickly, you should fill out the research details in the given fields on the hypothesis generator.

Below are the fields you should complete to generate your hypothesis:

• Who or what is your research based on? For instance, the subject can be research group 1.
• What does the subject (research group 1) do?
• What does the subject affect? - This shows the predicted outcome, which is the object.
• Who or what will be compared with research group 1? (research group 2).

Once you fill the in the fields, you can click the ‘Make a hypothesis’ tab and get your results.

⚗️ What Is a Hypothesis in the Scientific Method?

A hypothesis is a statement describing an expectation or prediction of your research through observation.

It is similar to academic speculation and reasoning that discloses the outcome of your scientific test . An effective hypothesis, therefore, should be crafted carefully and with precision.

A good hypothesis should have dependent and independent variables . These variables are the elements you will test in your research method – it can be a concept, an event, or an object as long as it is observable.

You can observe the dependent variables while the independent variables keep changing during the experiment.

In a nutshell, a hypothesis directs and organizes the research methods you will use, forming a large section of research paper writing.

Hypothesis vs. Theory

A hypothesis is a realistic expectation that researchers make before any investigation. It is formulated and tested to prove whether the statement is true. A theory, on the other hand, is a factual principle supported by evidence. Thus, a theory is more fact-backed compared to a hypothesis.

Another difference is that a hypothesis is presented as a single statement , while a theory can be an assortment of things . Hypotheses are based on future possibilities toward a specific projection, but the results are uncertain. Theories are verified with undisputable results because of proper substantiation.

When it comes to data, a hypothesis relies on limited information , while a theory is established on an extensive data set tested on various conditions.

You should observe the stated assumption to prove its accuracy.

Since hypotheses have observable variables, their outcome is usually based on a specific occurrence. Conversely, theories are grounded on a general principle involving multiple experiments and research tests.

This general principle can apply to many specific cases.

The primary purpose of formulating a hypothesis is to present a tentative prediction for researchers to explore further through tests and observations. Theories, in their turn, aim to explain plausible occurrences in the form of a scientific study.

It would help to rely on several criteria to establish a good hypothesis. Below are the parameters you should use to analyze the quality of your hypothesis.

🧭 6 Steps to Making a Good Hypothesis

Writing a hypothesis becomes way simpler if you follow a tried-and-tested algorithm. Let’s explore how you can formulate a good hypothesis in a few steps:

Step #1: Ask Questions

The first step in hypothesis creation is asking real questions about the surrounding reality.

Why do things happen as they do? What are the causes of some occurrences?

Your curiosity will trigger great questions that you can use to formulate a stellar hypothesis. So, ensure you pick a research topic of interest to scrutinize the world’s phenomena, processes, and events.

Step #2: Do Initial Research

Carry out preliminary research and gather essential background information about your topic of choice.

The extent of the information you collect will depend on what you want to prove.

Your initial research can be complete with a few academic books or a simple Internet search for quick answers with relevant statistics.

Still, keep in mind that in this phase, it is too early to prove or disapprove of your hypothesis.

Step #3: Identify Your Variables

Now that you have a basic understanding of the topic, choose the dependent and independent variables.

Take note that independent variables are the ones you can’t control, so understand the limitations of your test before settling on a final hypothesis.

Step #4: Formulate Your Hypothesis

You can write your hypothesis as an ‘if – then’ expression . Presenting any hypothesis in this format is reliable since it describes the cause-and-effect you want to test.

For instance: If I study every day, then I will get good grades.

Step #5: Gather Relevant Data

Once you have identified your variables and formulated the hypothesis, you can start the experiment. Remember, the conclusion you make will be a proof or rebuttal of your initial assumption.

So, gather relevant information, whether for a simple or statistical hypothesis, because you need to back your statement.

Step #6: Record Your Findings

Finally, write down your conclusions in a research paper .

Outline in detail whether the test has proved or disproved your hypothesis.

Edit and proofread your work, using a plagiarism checker to ensure the authenticity of your text.

We hope that the above tips will be useful for you. Note that if you need to conduct business analysis, you can use the free templates we’ve prepared: SWOT , PESTLE , VRIO , SOAR , and Porter’s 5 Forces .

❓ Hypothesis Formulator FAQ

Updated: Oct 25th, 2023

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Use our hypothesis maker whenever you need to formulate a hypothesis for your study. We offer a very simple tool where you just need to provide basic info about your variables, subjects, and predicted outcomes. The rest is on us. Get a perfect hypothesis in no time!

Crime Analysis for Problem Solvers in 60 Small Steps

Step 20: formulate hypotheses.

Whenever we confront some new and perplexing crime pattern we form hypotheses about its causes, often based on incomplete information. Experience and theory are good sources of hypotheses. You should (1) clearly state your hypotheses, (2) not be wedded to them, and (3) use data to objectively test them. Expect all hypotheses to be altered or discarded once relevant data have been examined because no hypothesis is completely right. For this reason it is often best to test multiple conflicting hypotheses.

A set of hypotheses is a roadmap for analysis. Hypotheses suggest types of data to collect, how this data should be analyzed, and how to interpret analysis results. If you were investigating drinking-related assaults in bars you might begin with the question, "How many bars are problem locations?" Based on the 80-20 rule (Step 18), you would state the hypothesis that some bars will have many fights, but most will have few or none. You would then test this hypothesis by listing the licensed drinking places and counting the number of assault reports at each over the last 12 months.

If your hypothesis was supported, you might ask the question, "What is different about the bars with many fights compared to the bars with few assaults?" The concept of risky facilities (Step 28) would help you form a set of three hypotheses:

• Risky bars have more customers.
• Risky bars have features that attract assaulters.
• Bar staff in risky bars either fail to control behaviors, or provoke fights.

You can test these hypotheses by gathering data on the number of customers at high- and low-risk bars, analyzing the number and rate of assaults per customer, observing the interactions of people at troublesome and trouble-free bars, and interviewing staff and customers.

If your first hypothesis was contradicted by the data, and you found that there was no great difference in numbers of assaults across drinking establishments, then you might ask the question, why are so many bars troublesome? This suggests another hypothesis: It's a perception problem; the city has about as many bar assaults as other comparable cities. This hypothesis suggests that you will need data from comparable cities.

If, after you collected the relevant data, you found that your city has an abnormally high number of problem bars, you might ask the question, "What is common to most bars in the city that produces a large number of assaults?" One hypothesis is that it is the way liquor licenses are dispensed and bars regulated. Another hypothesis is that there is something about the nature of bar customers in your city. Testing each would require you to collect relevant data and assess the validity of the hypothesis.

Notice how the questions and hypotheses structure the analysis. Test results - positive or negative - reveal new, more specific questions. The objective is to start with broad questions and hypotheses and, through a pruning process, come to a set of highly focused questions that point to possible responses.

Hypotheses suggest the type of data to collect. In the bar assaults example, the test of each hypothesis requires specific data. Sometimes the same data can test multiple hypotheses (as is the case with choosing among the three alternative explanations for risky bars). Often a variety of data is required to select among alternative hypotheses (as is the case with the last set of hypotheses). The more specific your hypotheses, the more focused your data collection will be. This is why it is more important to have a clear hypothesis you personally dislike, than an unclear hypothesis you approve of, or worse, no hypothesis at all.

Paralysis by Analysis

The lack of explicit hypotheses can lead to "paralysis by analysis," collecting too much data, conducting too much analysis, and not coming to any useful conclusion.

Hypotheses can help direct the analysis of data. Every clear hypothesis suggests a pattern of data that you should be able to observe, if the hypothesis is correct. In the example above, the hypotheses derived from the concept of risky facilities can be tested using a simple analytical procedure. If a bar is a crime generator, then you should see a high number of assaults, a high number of customers, but a low assault rate (see Step 17). Failure to find this pattern suggests the hypothesis is wrong. So it is important to have a clear idea of what you should observe if your hypothesis is correct, and what you should observe if your hypothesis is wrong (see third column of the table). If you cannot do this, then this is an indicator that your hypothesis may be too vague.

Hypotheses help interpret the analysis results. Let's assume that the analysis of bar fights showed that a few bars had most fights, and observations of the high- and low-risk bars indicated that the security staff of the risky bars provoked fights. This immediately suggests a possible avenue for intervention. In short, the validity of a hypothesis must make a difference. That is, if the hypothesis is true you will take a different decision than if it is false. If you will make the same decision regardless of the test results, then the hypothesis and its test are irrelevant.

In summary, hypotheses are important for guiding analysis. To formulate hypotheses you need to ask important questions, then create simple and direct speculative answers to these questions. These answers are your hypotheses. These speculations must be bold enough that they could be wrong, and there must be a way of showing whether they are right or wrong. If possible, create competing hypotheses.

Hypothesis formation is a useful group exercise, as it allows participants with contrary views to put their perspectives on the table in a way that allows clear and objective tests. In this way, participants contributing invalid hypotheses make substantial contributions to the analysis of the problem. If each hypothesis is linked to a potential solution, the test of these hypotheses simultaneously directs attention to feasible responses and rules out ineffective approaches.

Questions, Hypotheses, and Tests

• Acknowledgements

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UK competition watchdog steps up scrutiny of big tech’s role in AI startups

Microsoft and Amazon asked to comment on tie-ups, a move that paves way for formal investigation

The UK competition watchdog has stepped up its scrutiny of big tech involvement in artificial intelligence startups, asking for comment on three deals by Microsoft and Amazon.

The Competition and Markets Authority (CMA) announced that it was examining Microsoft’s investment in the French firm Mistral and the hiring of the DeepMind co-founder Mustafa Suleyman as head of the US company’s new AI division. The watchdog is also scrutinising Amazon’s $4bn (£3.2bn) investment in the US AI firm Anthropic .

The CMA has issued “invitations to comment” on the tie-ups, a procedural move that paves the way for a formal investigation, amid concerns that these partnerships are effectively giving the big tech companies backdoor control over potential rivals and stifling competition. The watchdog has already asked for comments on Microsoft’s relationship with OpenAI , the developer of ChatGPT.

Joel Bamford, the executive director of mergers at the CMA, said: “We will assess, objectively and impartially, whether each of these three deals fall within UK merger rules and, if they do, whether they have any impact on competition in the UK.”

Meanwhile, Sarah Cardell, the chief executive of the CMA, has warned that the watchdog had “real concerns” about the AI market . The CMA has singled out six tech companies at the heart of an “interconnected web” of AI partnerships: Google; Microsoft ; Meta; Amazon; Apple; and Nvidia, the leading supplier of chips for training and operating AI systems.

The CMA’s next step with Microsoft and Amazon will be a “phase one” investigation, during which the watchdog will examine whether the partnerships fall under the UK merger regime and whether they raise competition concerns. If the CMA finds there are concerns and decides to proceed with a phase two investigation, it could seek remedies from the companies involved.

In February, Microsoft announced it was investing €15m (£13m) in Mistral, a Paris-based startup specialising in open-source AI models, which can be freely downloaded and adapted by users. Microsoft said the deal represented an opportunity for Mistral to “unlock new commercial opportunities” and “expand to global markets”.

The Mistral deal was followed weeks later by the announcement of Suleyman’s appointment. Several employees at his Inflection AI startup were also recruited to join the new Microsoft division. The tie-up included a $650m cash payment to Inflection and making the company’s AI models available on Microsoft’s Azure cloud service.

Amazon’s deal with Anthropic , the developer of the Claude chatbot, involves the US startup using the big tech company as its main cloud provider and using Amazon’s custom chips to build, train and deploy AI models.

Alex Haffner, a partner at the UK law firm Fladgate, said the move underlined the CMA’s interest in the AI market. “Noises coming out of the CMA in recent times have indicated that they are very keen to get involved early in the evolution of the AI market and today’s announcement certainly reflects that degree of seriousness,” he said.

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Microsoft said it would provide the CMA with the information the watchdog needed to carry out its inquiries. “We remain confident that common business practices such as the hiring of talent or making a fractional investment in an AI startup promote competition and are not the same as a merger,” the company said.

Amazon said the CMA move was “unprecedented” and that the Anthropic deal was different from other tie-ups announced by the big tech firms. In a pointed reference to Microsoft’s partnership with OpenAI, Amazon said the deal did not give it a “board director or observer role” and continued to have “Anthropic running its models on multiple cloud providers”.

• Mergers, acquisitions and funding
• Competition and Markets Authority
• Artificial intelligence (AI)

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Buying bigger packs may not get best deal in UK supermarkets, says watchdog

UK has real concerns about AI risks, says competition regulator

Iceland boss hits out at parent ‘exploitation’ in baby milk market

‘Miss AI’ is billed as a leap forward – but feels like a monumental step backwards

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Biden Administration Releases Revised Title IX Rules

The new regulations extended legal protections to L.G.B.T.Q. students and rolled back several policies set under the Trump administration.

By Zach Montague and Erica L. Green

Reporting from Washington

The Biden administration issued new rules on Friday cementing protections for L.G.B.T.Q. students under federal law and reversing a number of Trump-era policies that dictated how schools should respond to cases of alleged sexual misconduct in K-12 schools and college campuses.

The new rules, which take effect on Aug. 1, effectively broadened the scope of Title IX, the 1972 law prohibiting sex discrimination in educational programs that receive federal funding. They extend the law’s reach to prohibit discrimination and harassment based on sexual orientation and gender identity, and widen the range of sexual harassment complaints that schools will be responsible for investigating.

“These regulations make it crystal clear that everyone can access schools that are safe, welcoming and that respect their rights,” Miguel A. Cardona, the education secretary, said in a call with reporters.

The rules deliver on a key campaign promise for Mr. Biden, who declared he would put a “quick end” to the Trump-era Title IX rules and faced mounting pressure from Democrats and civil rights leaders to do so.

The release of the updated rules, after two delays, came as Mr. Biden is in the thick of his re-election bid and is trying to galvanize key electoral constituencies.

Through the new regulations, the administration moved to include students in its interpretation of Bostock v. Clayton County, the landmark 2020 Supreme Court case in which the court ruled that the Civil Rights Act of 1964 protects gay and transgender workers from workplace discrimination. The Trump administration held that transgender students were not protected under federal laws, including after the Bostock ruling .

In a statement, Betsy DeVos, who served as Mr. Trump’s education secretary, criticized what she called a “radical rewrite” of the law, asserting that it was an “endeavor born entirely of progressive politics, not sound policy.”

Ms. DeVos said the inclusion of transgender students in the law gutted decades of protections and opportunities for women. She added that the Biden administration also “seeks to U-turn to the bad old days where sexual misconduct was sent to campus kangaroo courts, not resolved in a way that actually sought justice.”

While the regulations released on Friday contained considerably stronger protections for L.G.B.T.Q. students, the administration steered clear of the lightning-rod issue of whether transgender students should be able to play on school sports teams corresponding to their gender identity.

The administration stressed that while, writ large, exclusion based on gender identity violated Title IX, the new regulations did not extend to single-sex living facilities or sports teams. The Education Department is pursuing a second rule dealing with sex-related eligibility for male and female sports teams. The rule-making process has drawn more than 150,000 comments.

Under the revisions announced on Friday, instances where transgender students are subjected to a “hostile environment” through bullying or harassment, or face unequal treatment and exclusion in programs or facilities based on their gender identity, could trigger an investigation by the department’s Office for Civil Rights.

Instances where students are repeatedly referred to by a name or pronoun other than one they have chosen could also be considered harassment on a case-by-case basis.

“This is a bold and important statement that transgender and nonbinary students belong, in their schools and in their communities,” said Olivia Hunt, the policy director for the National Center for Transgender Equality.

The regulations appeared certain to draw to legal challenges from conservative groups.

May Mailman, the director of the Independent Women’s Law Center, said in a statement that the group planned to sue the administration. She said it was clear that the statute barring discrimination on the basis of “sex” means “binary and biological.”

“The unlawful omnibus regulation reimagines Title IX to permit the invasion of women’s spaces and the reduction of women’s rights in the name of elevating protections for ‘gender identity,’ which is contrary to the text and purpose of Title IX,” she said.

The existing rules, which took effect under Mr. Trump in 2020, were the first time that sexual assault provisions were codified under Title IX. They bolstered due process rights of accused students, relieved schools of some legal liabilities and laid out rigid parameters for how schools should conduct impartial investigations.

They were a sharp departure from the Obama administration’s interpretation of the law, which came in the form of unenforceable guidance documents directing schools to ramp up investigations into sexual assault complaints under the threat of losing federal funding. Scores of students who had been accused of sexual assault went on to win court cases against their colleges for violating their due process rights under the guidelines.

The Biden administration’s rules struck a balance between the Obama and Trump administration’s goals. Taken together, the regulation largely provides more flexibility for how schools conduct investigations, which advocates and schools have long lobbied for.

Catherine E. Lhamon, the head of the department’s Office for Civil Rights who also held the job under President Barack Obama, called the new rules the “most comprehensive coverage under Title IX since the regulations were first promulgated in 1975.”

They replaced a narrower definition of sex-based harassment adopted under the Trump administration with one that would include a wider range of conduct. And they reversed a requirement that schools investigate only incidents alleged to have occurred on their campuses or in their programs.

Still, some key provisions in the Trump-era rules were preserved, including one allowing informal resolutions and another prohibiting penalties against students until after an investigation.

Among the most anticipated changes was the undoing of a provision that required in-person, or so-called live hearings, in which students accused of sexual misconduct, or their lawyers, could confront and question accusers in a courtroom-like setting.

The new rules allow in-person hearings, but do not mandate them. They also require a process through which a decision maker could assess a party or witness’s credibility, including posing questions from the opposing party.

“The new regulations put an end to unfair and traumatic grievance procedures that favor harassers,” Kel O’Hara, a senior attorney at Equal Rights Advocates. “No longer will student survivors be subjected to processes that prioritize the interests of their perpetrators over their own well being and safety.”

The new rules also allow room for schools to use a “preponderance of evidence” standard, a lower burden of proof than the DeVos-era rules encouraged, through which administrators need only to determine whether it was more likely than not that sexual misconduct had occurred.

The renewed push for that standard drew criticism from legal groups who said the rule stripped away hard-won protections against flawed findings.

“When you are dealing with accusations of really one of the most heinous crimes that a person can commit — sexual assault — it’s not enough to say, ‘50 percent and a feather,’ before you brand someone guilty of this repulsive crime,” said Will Creeley, the legal director of the Foundation for Individual Rights and Expression.

The changes concluded a three-year process in which the department received 240,000 public comments. The rules also strengthen protections for pregnant students, requiring accommodations such as a bigger desk or ensuring access to elevators and prohibiting exclusion from activities based on additional needs.

Title IX was designed to end discrimination based on sex in educational programs or activities at all institutions receiving federal financial assistance, beginning with sports programs and other spaces previously dominated by male students.

The effects of the original law have been pronounced. Far beyond the impact on school programs like sports teams, many educators credit Title IX with setting the stage for academic parity today. Female college students routinely outnumber male students on campus and have become more likely than men of the same age to graduate with a four-year degree.

But since its inception, Title IX has also become a powerful vehicle through which past administrations have sought to steer schools to respond to the dynamic and diverse nature of schools and universities.

While civil rights groups were disappointed that some ambiguity remains for the L.G.B.T.Q. students and their families, the new rules were widely praised for taking a stand at a time when education debates are reminiscent to the backlash after the Supreme Court ordered schools to integrate.

More than 20 states have passed laws that broadly prohibit anyone assigned male at birth from playing on girls’ and women’s sports teams or participating in scholastic athletic programs, while 10 states have laws barring transgender people from using bathrooms based on their gender identity.

“Some adults are showing up and saying, ‘I’m going to make school harder for children,” said Liz King, senior program director of the education equity program at the Leadership Conference on Civil and Human Rights. “It’s an incredibly important rule, at an incredibly important moment.”

Schools will have to cram over the summer to implement the rules, which will require a retraining staff and overhauling procedures they implemented only four years ago.

Ted Mitchell, the president of the American Council on Education, which represents more than 1,700 colleges and universities, said in a statement that while the group welcomed the changes in the new rule, the timeline “disregards the difficulties inherent in making these changes on our nation’s campuses in such a short period of time.”

“After years of constant churn in Title IX guidance and regulations,” Mr. Mitchell said, “we hope for the sake of students and institutions that there will be more stability and consistency in the requirements going forward.”

Zach Montague is based in Washington. He covers breaking news and developments around the district. More about Zach Montague

Erica L. Green is a White House correspondent, covering President Biden and his administration. More about Erica L. Green